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Molecular Gut Analysis of Carabids (Coleoptera: carabidae) using Aphid Primers: Author: Wallace, Sarah Kate; Date: 2004-08-15; Program: Entomology; Abstract: Carabid beetles are found in a variety of Montana crops, although their impact on the food web is poorly known. To detect aphidophagy using the polymerase chain reaction (PCR), carabids were fed a single aphid and allowed to digest for increasing time intervals. Aphidspecific PCR primers were used to amplify aphid DNA from carabid beetles. For the laboratory-fed beetles, PCR detection of aphidophagy decreased with longer digestion periods. Further, there were differences among genera in the proportion of fed beetles positive for aphid DNA at the tested digestion intervals. Field surveys of 273...; Download File | View full details