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An Analysis of Dendritic Cooperativity in Protein Hydrolysis: Author: O'Dell, Jacob Webb; Date: 2005-08-15; Program: Chemistry; Abstract: Catalysts are compounds that increase the rate of a chemical reaction by lowering the activation energy while not being permanently altered. Adding a catalyst to a reaction, while increasing the rate, complicates purification because the catalyst must be separated from the product(s) of the reaction. If the catalyst is a drastically different size than the product then seperation can be achieved as easily as filtering over a membrane. Dendrimers are becoming popular scaffolding for tethering catalysts. Attaching a catalyst to a dendrimer makes a bigger catalytic unit that is easily separated f...; Download File | View full details

The Synthesis of Glycodendrimers and their Applications in Carbohydrate-Protein Interactions and Catalysis: Author: Morgan, Joel Ryan; Date: 2006-08-15; Program: Chemistry; Abstract: Glycodendrimers were synthesized for studies in carbohydrate-protein interactions, and from these studies we gained insight into the mechanism of some carbohydrate-protein interactions. First, the synthesis of tris-mannose cluster functionalized glycodendrimers of different sizes using a "click chemistry" approach is described. The macromolecules were characterized using 1H and 13C NMR and MALDITOF MS. Next, a heterogeneous dendrimer functionalization strategy to control the presentation of the carbohydrate clusters around the dendrimer periphery is reported. Nacetyl galactosamine an...; Download File | View full details

Mannose/TEMPO Functionalized PAMAM Dendrimers: Their Relative Locations and Components of Affinity Toward Concanavalin A: Author: Samuelson, Lynn Elizabeth; Date: 2004-05-15; Program: Chemistry; Abstract: Surface functionalized dendrimers are being used for several applications including the study of protein-carbohydrate interactions. Mannose-functionalized dendrimers with varying concentrations of saccharides on the dendrimer surface were synthesized. Spin labels (2,2,6,6-tetramethylpiperidine N-oxide) were incorporated onto the dendrimer's surface as well. Linebroadening effects in the EPR spectra of these compounds allowed us to determine the distance between spin labels (and thus between carbohydrates). The mannose-spin labeled functionalized dendrimers were further studied to determine eff...; Download File | View full details

The use of In(OTf)3 as a Lewis Acid in carbohydrate chemistry, and exploration in silicon tethered reactions: Author: Bizier, Nicholas Paul; Date: 2008-12-15; Program: Chemistry; Abstract: The feasibility of using Indium (III) triflate as a Lewis Acid for a number of different carbohydrate reactions was explored. First, the use of In(OTf)3 as a catalyst in the acylation of a number of carbohydrates was explored. The utility and compatibility of the In(OTf)3 reaction conditions with a number of protecting groups on the sugars were examined by 1H NMR analysis. In the second part of the carbohydrate methodology, the feasibility of using In(OTf)3 to promote glycosylation reactions using a number of glycosyl donors was evaluated. These studies were conducted using 1H NMR, 13CNMR, and...; Download File | View full details

Using PAMAM dendrimer frameworks to investigate multivalent binding in protein-carbohydrate interactions: Author: Wolfenden, Mark Leroy; Date: 2009-08-15; Program: Chemistry; Abstract: Polyvalent interactions in biological systems have been of great interest recently; how nature creates high affinity polyvalent binding with low monomeric affinity, is yet to be clearly understood. We have created a bivalent lectincarbohydrate binding system using dendrimers as the carbohydrate mounted scaffold and Concanavalin A (Con A) as the mannose/glucose binding lectin to investigate this mode of interaction. The relative affinities of the utilized carbohydrates toward Con A are: mannose binds 4 times stronger than glucose, and galactose shows no affinity. With these relative affinities ...; Download File | View full details