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Protein-Protein interactions involved in the biogenesis of eukaryotic small ribosomal subunits: Author: Castle, Cathy Lynn; Date: 2008-12-15; Program: Microbiology; Abstract: Ribosome biogenesis is a complicated process involving numerous proteins and modification factors. The process has been well-documented in prokaryotic cells where it is much less complex than the process involved in eukaryotic cells. In eukaryotes, much of what is known about ribosome biogenesis has been learned from studies in the yeast Saccharomyces cerevisiae. Far less has been learned about higher eukaryotes such as humans. However, among organisms in all three domains of life, ribosome structure and function is well conserved. The biogenesis of ribosomal subunits is dynamic, complicated, ...; Download File | View full details

DNA binding proteins of archaeal viruses: Author: Geary, Joella Suzanne; Date: 2008-12-15; Program: Microbiology; Abstract: Archaea are single-celled organisms comprising the third domain of life. The Achaeal species Sulfolobus are infected by the Fuselloviridae virus family: SSV1, SSV2, SSV-RH, and SSV-K. The genomes of these viruses have been annotated and contain putative DNA-binding proteins. The purpose of this work is to identify DNA sequences bound by the SSV1 putative DNA-binding protein C43. C43 protein was cloned, expressed, purified, and assayed at various temperatures for interaction with three SSV1 DNA sequences. C43 binds the T5-promoter, T6-promoter, and C43-promoter sequentially and consistently. Ad...; Download File | View full details