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Your search for ETD Program Veterinary Molecular Biology resulted in 15 match(es).

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Comprehensive Transcriptional Profiling of γδ T Cells: Author: Graff, Jill Christin; Date: 2005-12-15; Program: Veterinary Molecular Biology; Abstract: γδ T cells have been conserved since the adaptive immune system arose, yet their importance is still unclear. In an attempt to compensate for the lack of a broad knowledge-base of γδ T cells across species, global analyses of γδ T cell transcriptomes have been performed using serial analysis of gene expression (SAGE). Twelve new SAGE libraries were generated from the following bovine lymphocyte populations: magnetic bead-sorted blood γδ T cells, spleen γδ T cells and enr...; Download File | View full details

Identification of Novel Virulence Factors and Mechanisms of Pathogenesis from the Sexually Transmitted Protozoan Tritrichomonas foetus: Author: Higgins, Melanie Rae; Date: 2006-05-15; Program: Veterinary Molecular Biology; Abstract: Tritrichomonas foetus (T. foetus) is the cause of trichomoniasis in cattle. Little is known regarding the basis of virulence in this parasite. Early host-parasite interactions most likely affect the ability of the parasite to colonize the female reproductive tract. These early interactions shape the course of disease and ultimately control the outcome of pathogenesis. The aim of the studies herein is to provide insight into the factors that alter the progression of trichomoniasis. We examined how environmental stress and estradiol treatment affect pathogenesis of trichomoniasis. Acute T. foetu...; Download File | View full details

Mid-and Late-Gestation Lethality in Mice Lacking the N Terminus of TATA-Binding Protein: Author: Hobbs, Nicole Kay; Date: 2004-12-15; Program: Veterinary Molecular Biology; Abstract: TATA-binding protein (TBP) is a transcription factor comprised of a 180 amino acid core that is shared by all eukaryotes. TBP also has an N-terminal region that, in vertebrates, is highly conserved. We have generated mice bearing a mutant tbp allele, tbp∆N, that lacks 111 of the 135 amino acids of the vertebrate specific N terminus. Most homozygous mutants, tbp∆N/∆N, die at midgestation from an apparent defect in their placentas. tbp∆N/∆N fetuses were rescued at this midgestational crisis if supplied with a wild-type tetraploid placenta. tbp∆N/&a...; Download File | View full details

Molecular and Functional Characterization of Bovine C5a Receptor: Author: Nemali, Sailasree; Date: 2006-05-15; Program: Veterinary Molecular Biology; Abstract: Anaphylatoxin C5a is an important chemotactic factor for bovine neutrophils, and is the earliest inflammatory agent formed during bovine mastitis. Bovine neutrophils respond to C5a and its truncated form C5a des arg with similar affinities unlike human neutrophils. Therefore, to test the hypothesis that the bovine C5a receptor structure and signaling differ from that of the human C5a receptor, we cloned and analyzed the bovine C5a receptor. In the present investigation, the bovine C5a receptor encoding cDNA from bovine bone marrow was cloned and the recombinant C5a receptor protein was express...; Download File | View full details

The Role of VPg in Translation of Calicivirus RNA: Author: Daughenbaugh, Katie Finney; Date: 2005-12-15; Program: Veterinary Molecular Biology; Abstract: Molecular mechanisms of Norovirus replication remain for the most part undefined, primarily due to the lack of cell culture and small animal model systems. However, sequence comparisons and studies using cultivable caliciviruses have lead to the description of many features of the viral genome. Genomes are positive sense RNA, where the genome itself serves as mRNA for the production of viral protein. Additionally, viral RNA is covalently attached at the 5' end to the viral protein VPg. VPg is required for infectivity of the RNA by transfection, and removal of VPg by proteinase K treatment redu...; Download File | View full details

Identification and Characterization of Novel Protein-Protein Interactions with the Basal Transcription Factor, TATA-Binding Protein: Author: Prigge, Justin Robert; Date: 2006-05-15; Program: Veterinary Molecular Biology; Abstract: Recruitment of the TATA-Binding Protein, TBP, to the promoter is a critical, rate-limiting step that drives the initial phase of nearly all gene transcription events. Because of this, many transcriptional regulators target TBP, either to localize TBP at the promoter, or to relay signals between other promoter-bound protein complexes and the basal transcription machinery. Studies described herein were designed to identify novel protein-protein interactions with TBP. To do this, we screened mid-gestational pregnancy-associated cDNA prey libraries using two different yeast two-hybrid systems. Scr...; Download File | View full details

Effects of a Primary Influenza Infection on Susceptibility to a Secondary Streptococcus pneumoniae Infection: Author: McNamee, Lynnelle Ann; Date: 2006-12-15; Program: Veterinary Molecular Biology; Abstract: Influenza infections result in increased susceptibility to a secondary Streptococcus pneumoniae infection. The aim of the present studies was to determine the mechanism(s) responsible for this increase in susceptibility. Using an in vivo co-infection model, we found that susceptibility to S. pneumoniae was significantly increased at 6 days but not 3 days after an influenza infection. We depleted mice of neutrophils and found that neutrophils were important in the response to S. pneumoniae in mice infected with bacteria only or those infected with influenza for 3 days prior to a S. pneumoniae i...; Download File | View full details

Loss of the Murine TATA-Binding Protein N Terminus Leads to Placental Labyrinth Defects but not Maternal Adaptive Immune Responses: Author: Sealey, Amy Lynn; Date: 2007-05-15; Program: Veterinary Molecular Biology; Abstract: The TATA-binding protein (TBP) is a component of the basal transcription machinery. The TBP C-terminal core is conserved in all eukaryotes, but the N terminal region is shared between vertebrates. To study this, we generated a mouse line lacking 111 of the 135 amino acids of the vertebrate-specific sequence. The mutant tbp allele was designated tbpΔN, and the majority of homozygous mutants, tbpΔN/ΔN, died at midgestation due to defects in the placenta. Previous studies in our laboratory showed that tbpΔN/ΔN fetuses survived the midgestational crisis if the mo...; Download File | View full details

Identification and Characterization of a Novel Transcription Factor that Regulates NCF2 Expression via the TNF-alpha Responsive Region: Author: Anderson, Mary Cloud Bosworth Ammons; Date: 2007-12-15; Program: Veterinary Molecular Biology; Abstract: The multicomponent NADPH oxidase is an essential enzyme complex found in professional phagocytic cells that mediates innate immune defence against multiple pathogens through the production of reactive oxygen species. The vital and functionally limiting cytosolic component of the NADPH oxidase, p67phox, is transcriptionally regulated by TNF-α at the TNF-α Responsive Region (TRR) in the intragenic region of Neutrophil Cytosolic Factor 2 (NCF2), the gene which codes for p67phox. The aim of this dissertation is to identify and charaterize the factor(s) that binds the TR...; Download File | View full details

Select Procyanidins induce gammadelta T cell activation and proliferation: Author: Holderness, Jeffery Scott; Date: 2008-05-15; Program: Veterinary Molecular Biology; Abstract: Many pharmaceutical drugs in use today were originally identified in plants from traditional medicine. However, there remain many plants in traditional medicine that produce confusing immune responses and are therefore unlikely candidates for pharmaceutical drugs. The effects of some of the traditional medicines that induce these confusing immune responses may now be explained by recent advances in the characterization of our immune system, namely in our understanding of the unique functions of the γδ T cell. These γδ T cell functions include t...; Download File | View full details

Rotavirus NSP1 is an interferon system antagonist: Author: Graff, Joel Wallace; Date: 2008-08-15; Program: Veterinary Molecular Biology; Abstract: Rotaviruses cause severe gastroenteritis in mammals, including humans and livestock. Most rotavirus proteins have known functions, either as a mediator of virus replication or as a component of the infectious virus particle. The function of nonstructural protein 1 (NSP1) was unknown. However, it has been suggested that the function of NSP1 involved interactions with cellular proteins. Using the NSP1 encoded by a bovine rotavirus as the bait protein of a yeast-two hybrid interaction trap, interferon regulatory factor 3 (IRF3) was identified as an NSP1-interacting protein. Due to the importance ...; Download File | View full details

Studies of gene expression control in the apicomplexan parasite Toxoplasma gondii: Author: Behnke, Michael Sean; Date: 2008-08-15; Program: Veterinary Molecular Biology; Abstract: Our understanding of global gene expression patterns and control of both developmental and strain specific aspects of Toxoplasma gondii has broadened in the past few years. A global approach was initially undertaken to construct the "transcriptome" for the Toxoplasma intermediate life cycle using serial-analysis-of-gene expression (SAGE). From this analysis, we confirmed the increased expression of known as well as novel mRNAs associated with the tachyzoite-to-bradyzoite transition. Accumulation of bradyzoite specific mRNAs in the bradyzoite SAGE libraries raises the possibility th...; Download File | View full details

Low dose tolerance vaccine platform, reovirus protein sigma 1, and treatment of autoimmunity: Author: Rynda, Agnieszka; Date: 2008-08-15; Program: Veterinary Molecular Biology; Abstract: Effective treatments for multiple sclerosis (MS) are problematic due to its unknown etiology. Experimental autoimmune encephalomyelitis (EAE) in rodents mimics MS. Mucosal treatment of EAE with antigens to induce tolerance is effective, but requires large and/or multiple administrations, which introduces an allergy risk. We utilized reovirus adhesin, protein sigma 1 (p sigma1), to improve mucosal auto-antigen delivery and show that a single low-dose of pσ1-based vaccines induces tolerance and prevents autoimmunity when administered nasally. We engineered three pσ1-based vaccines ca...; Download File | View full details

Molecular and systemic functions of the vertebrate-specific TATA-binding protein N terminus: Author: Lucas, Olivier; Date: 2009-05-15; Program: Veterinary Molecular Biology; Abstract: The invertebrate/vertebrate transition and associated innovations can be regarded as a major event in evolution. Recent Molecular progresses invite to an analysis of the events leading to the apparition of vertebrates and the underlying embellishment in gene regulation. In eukaryotes, the TATA-Binding Protein (TBP) has a central role in transcription initiation of most genes. TBP is comprised of a highly conserved DNA binding domain and, in vertebrates, it also contains a novel region: the N-terminal TBP protein coding sequence. The role of the TBP-N is largely unknown, but previous studies su...; Download File | View full details

Vaccine platform for infection or autoimmune diseases using an ETEC fimbrial scaffold: Author: Jun, SangMu; Date: 2009-05-15; Program: Veterinary Molecular Biology; Abstract: The expression of enterotoxigenic Escherichia coli (ETEC) fimbriae (colonization factor antigen I (CFA/I) or K99) on the surface of a Salmonella vaccine vector confers protection against ETEC challenge. Application of such fimbriae as a treatment for the proinflammatory disease, experimental autoimmune encephalomyelitis (EAE), or as a Molecular scaffold for heterologous antigen expression by cloning enterohemorrhagic E. coli (EHEC) LPS peptide mimetics into the K99 fimbriae to produce a dual vaccine for ETEC/EHEC was investigated. The expression of CFA/I fimbriae by a Salmonella vaccine vector...; Download File | View full details

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