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Characterization and isolation of Archael thermophilic hosts and viruses from Yellowstone National Park: Author: Spuhler, Joshua Lupine; Date: 2009-08-15; Program: Microbiology; Abstract: My research is focused on the identification and characterization of new archaeal viruses that inhabit the thermal features of Yellowstone National Park (YNP). I have undertaken the systematic survey of more than 90 different thermal features found in Yellowstone through a variety of means including culturing of hosts, MDA amplification, qPCR for known archaeal viruses, 16S rRNA gene analysis of potential resident archaeal hosts, tangential flow and end point filtration approaches to sample new viruses, and general water geochemical analysis. From this work a new host has been isolated from YN...; Download File | View full details